Plant regeneration from leaf mesophyll protoplasts of Lycopersicon glandulosum.
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PLANT REGENERATION FROM MESOPHYLL PROTOPLASTS OF POTATO (SOLANUM TUBEROSUM L.) CULTIVAR DELAWARE USING SILVER THIOSULFATE (STS)
The use of in vitro potato shoot cultures for protoplast isolation is desirable for consistent protoplast qualities. Ethylene build-up in the culture vessels causes problems for leaf growth (e.g. decreasing leaf surface, dry weight and chlorophyll) in shoot culture. Fifty to one hundred µM Silver thiosulfate (STS) produced a larger leaf area as a source of plant material for protoplast isolatio...
متن کاملMesophyll cell protoplasts of potato: isolation, proliferation, and plant regeneration.
Mesophyll cell protoplasts were isolated from potato (Solanum tuberosum L. cv. Russet Burbank) leaves and induced to proliferate in culture. Protoplast division was observed only among preparations isolated from plants previously conditioned under short periods of low intensity illumination. Sustained growth and development of protoplast-derived calli (p-calli) occurred when they were maintaine...
متن کاملplant regeneration from mesophyll protoplasts of potato (solanum tuberosum l.) cultivar delaware using silver thiosulfate (sts)
the use of in vitro potato shoot cultures for protoplast isolation is desirable for consistent protoplast qualities. ethylene build-up in the culture vessels causes problems for leaf growth (e.g. decreasing leaf surface, dry weight and chlorophyll) in shoot culture. fifty to one hundred µm silver thiosulfate (sts) produced a larger leaf area as a source of plant material for protoplast isolatio...
متن کاملPlant Regeneration from Mesophyll Protoplasts of Potato (solanum Tuberosum L.) Cultivar Delaware Using Silver Thiosulfate (sts)
The use of in vitro potato shoot cultures for protoplast isolation is desirable for consistent protoplast qualities. Ethylene build-up in the culture vessels causes problems for leaf growth (e.g. decreasing leaf surface, dry weight and chlorophyll) in shoot culture. Fifty to one hundred μM Silver thiosulfate (STS) produced a larger leaf area as a source of plant material for protoplast isolatio...
متن کاملRegeneration from leaf protoplasts of Arabidopsis thaliana ecotype estland.
Protoplasts (2 x 10(7)/g fresh wt) were isolated from leaves of A. thaliana ecotype estland, with a viability of more than 90%. Protoplasts cultured in calcium alginate beads or layers showed division while culture in liquid or agarose beads failed to elicit any division. Effect of culture density showed highest frequency of division occurring at 5 x 10(5) while no division was seen when cultur...
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ژورنال
عنوان ژورنال: Plant tissue culture letters
سال: 1989
ISSN: 1884-7706,0289-5773
DOI: 10.5511/plantbiotechnology1984.6.155